Evaluación de la presencia de bacteriófagos provenientes de aguas residuales y suelos compostados

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dc.contributor.advisorGaviria Arias, Duverney
dc.contributor.authorOcampo Ramírez, Valentina
dc.contributor.authorGiraldo Otalvaro, María Camila
dc.coverage.spatialPereiraspa
dc.creator.emailokvalen12@gmail.comspa
dc.date.accessioned2024-01-18T19:42:39Z
dc.date.available2024-01-18T19:42:39Z
dc.date.created2023-12-04
dc.description.abstractObjetivo: Determinar la presencia de bacteriófagos; con capacidad lítica contra bacterias ESKAPE, provenientes de suelos compostados y aguas residuales pertenecientes a la universidad Libre Método: Se realizó una investigación en campo, experimental con enfoque cuantitativo, utilizando como sustratos el suelo de la compostera de universidad Libre seccional Pereira y el agua de la quebrada “La Mielita”. para realizar la toma de muestras, aislamiento y titulación de los bacteriófagos fue tomado como referencia el libro Bacteriophages Methods and Protocols, Volume 1: Isolation, Characterization, and Interactions. (1) Resultados: En la investigación se logró aislar dos bacteriófagos con capacidades líticas contra Klebsiella spp y E. coli, en agua residual y suelo compostado respectivamente; de determinó un título de 4x107 unidades formadoras de placa / ml para el fago con la capacidad de lisar E. coli en suelo compostado. Para el fago con la capacidad de lisar Klebsiella spp en suelo compostado, se establece un título de 4x103 unidades formadoras de placa / ml. Así mismo fue posible establecer un método para obtener los bacteriófagos y establecer la bacteria hospedadora del fago y su capacidad líticaspa
dc.description.abstractenglishObjective: To determine the presence of bacteriophages; with lytic capacity against ESKAPE bacteria, from composted soils and wastewater belonging to the Universidad Libre. Method: An experimental field research was carried out with a quantitative approach, using as substrates the soil from the compost bin of the Universidad Libre seccional Pereira and the water from the "La Mielita" stream. The book Bacteriophages Methods and Protocols, Volume 1: Isolation, Characterization, and Interactions was used as a reference for the sampling, isolation and titration of bacteriophages (1). Results: Two bacteriophages with lytic capacities against Klebsiella spp and E. coli were isolated in wastewater and composted soil, respectively; a titer of 4x107 plaque-forming units/ml was determined for the phage with the capacity to lyse E. coli in composted soil. For the phage with the ability to lyse Klebsiella spp in composted soil, a titer of 4x103 plaque-forming units/ml was established. It was also possible to establish a method to obtain the bacteriophages and to establish the host bacteria of the phage and its lytic capacity.spa
dc.description.sponsorshipUniversidad Libre Seccional Pereira -- Facultad de Ciencias de la Salud, Exactas y Naturales -- Microbiologíaspa
dc.formatPDFspa
dc.identifier.urihttps://hdl.handle.net/10901/27960
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dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.coarhttp://purl.org/coar/access_right/c_abf2spa
dc.rights.licenseAtribución-NoComercial-SinDerivadas 2.5 Colombiaspa
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.5/co/spa
dc.subjectBacteriófagosspa
dc.subjectFarmacorresistencia Microbianaspa
dc.subjectTerapia de Fagosspa
dc.subjectBacteriólisisspa
dc.subject.subjectenglishBacteriophagesspa
dc.subject.subjectenglishMicrobial Drug Resistancespa
dc.subject.subjectenglishPhage Therapyspa
dc.subject.subjectenglishBacteriolysisspa
dc.titleEvaluación de la presencia de bacteriófagos provenientes de aguas residuales y suelos compostadosspa
dc.title.alternativeEvaluation of the presence of bacteriophages from wastewater and composted soils.spa
dc.type.driverinfo:eu-repo/semantics/bachelorThesisspa
dc.type.hasversioninfo:eu-repo/semantics/acceptedVersionspa
dc.type.localTesis de Pregradospa

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